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Alex J Hughes
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Assistant Professor of Bioengineering in Cell and Developmental Biology
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Department: Cell and Developmental Biology
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Contact information
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210 S. 33rd St.
3d Suite 240, Skirkanich Hall
Philadelphia, PA 19104
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3d Suite 240, Skirkanich Hall
Philadelphia, PA 19104
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Office: 2155735233
3c Lab: 2155736260
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3c Lab: 2155736260
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Email:
ajhughes@seas.upenn.edu
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ajhughes@seas.upenn.edu
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Education:
21 7 BE 2d (Chemical & Materials Engineering) c
2f University of Auckland, 2008.
21 8 BSc 19 (Pharmacology) c
2f University of Auckland, 2008.
21 8 PhD 1b (Bioengineering) c
3b University of California, Berkeley, 2013.
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Permanent link21 7 BE 2d (Chemical & Materials Engineering) c
2f University of Auckland, 2008.
21 8 BSc 19 (Pharmacology) c
2f University of Auckland, 2008.
21 8 PhD 1b (Bioengineering) c
3b University of California, Berkeley, 2013.
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17e Our lab works to bring developmental processes that operate in vertebrate embryos and regenerating organs under an engineering control framework, so that we can build better tissues. We study how cells conspire to create complex structures, so that we can build models of human disease, reconstituted tissues for regenerative medicine, and new types of biological machines.
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10 Keywords
7b Developmental engineering, epithelial morphogenesis, microfluidics, mechanics of development, kidney organogenesis.
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18 Research Details
106 Many diseases arise from a breakdown in tissue structure, including cancers and congenital birth defects. We aim to reverse engineer the interactions between cells and their local environment that create and maintain the structure of tissues in the body.
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120 We are particularly interested in learning to control patterning processes that occur in embryos, where the blueprint of adult tissues is established. We approach this by developing new cellular imaging, tissue construction, soft materials, and micro-scale engineering techniques.
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1b7 Project areas: engineered shape-shifting soft materials including tissue scaffolds that impose developmentally relevant mechanical strains to study and synthetically drive morphogenesis, microfluidic devices for large-scale production of compositionally controlled organoids, single-cell proteomics using sequence-able antibody tag strategies, multiplexed cell patterning to synthetically reconstitute planar cell polarity dynamics.
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Description of Research Expertise
23 Research Interests17e Our lab works to bring developmental processes that operate in vertebrate embryos and regenerating organs under an engineering control framework, so that we can build better tissues. We study how cells conspire to create complex structures, so that we can build models of human disease, reconstituted tissues for regenerative medicine, and new types of biological machines.
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7b Developmental engineering, epithelial morphogenesis, microfluidics, mechanics of development, kidney organogenesis.
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18 Research Details
106 Many diseases arise from a breakdown in tissue structure, including cancers and congenital birth defects. We aim to reverse engineer the interactions between cells and their local environment that create and maintain the structure of tissues in the body.
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120 We are particularly interested in learning to control patterning processes that occur in embryos, where the blueprint of adult tissues is established. We approach this by developing new cellular imaging, tissue construction, soft materials, and micro-scale engineering techniques.
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1b7 Project areas: engineered shape-shifting soft materials including tissue scaffolds that impose developmentally relevant mechanical strains to study and synthetically drive morphogenesis, microfluidic devices for large-scale production of compositionally controlled organoids, single-cell proteomics using sequence-able antibody tag strategies, multiplexed cell patterning to synthetically reconstitute planar cell polarity dynamics.
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117 Hughes, AJ, Mornin, JD, Biswas, SK, Beck, LE, Bauer, DP, Raj, A, Bianco, S, Gartner, ZJ: Quanti.us: a tool for rapid, flexible, crowd-based annotation of images. nature methods 15(8): 587-+ AUG 2018.
13b Hughes, AJ, Miyazaki, H, Coyle, MC, Zhang, J, Laurie, MT, Chu, D, Vavrusova, Z, Schneider, RA, Klein, OD, Gartner, ZJ: Engineered Tissue Folding by Mechanical Compaction of the Mesenchyme. developmental cell 44(2): 165-+ JAN 22 2018.
f8 Hughes, AJ, Coyle, MC, Zhang, J, Gartner, ZJ: Tissue origami: Directed folding of tissues by programmed cell contractility networks. molecular biology of the cell 27, 2016.
126 Todhunter, ME, Jee, NY, Hughes, AJ, Coyle, MC, Cerchiari, A, Farlow, J, Garbe, JC, LaBarge, MA, Desai, TA, Gartner, ZJ: Programmed synthesis of three-dimensional tissues. nature methods 12(10): 975-981, OCT 2015.
d6 Hughes, AJ, Spelke, DP, Xu, ZC, Kang, CC, Schaffer, DV, Herr, AE: Single-cell western blotting. nature methods 11(7): 749-U94, JUL 2014.
100 Tentori, AM, Hughes, AJ, Herr, AE: Microchamber Integration Unifies Distinct Separation Modes for Two-Dimensional Electrophoresis. analytical chemistry 85(9): 4538-4545, MAY 7 2013.
f1 Hughes, AJ, Herr, AE: Microfluidic Western blotting. proceedings of the national academy of sciences of the united states of america 109(52): 21450-21455, DEC 26 2012.
138 Hughes, AJ, Tentori, AM, Herr, AE: Bistable Isoelectric Point Photoswitching in Green Fluorescent Proteins Observed by Dynamic Immunoprobed Isoelectric Focusing. journal of the american chemical society 134(42): 17582-17591, OCT 24 2012.
129 Hughes, AJ, Lin, RKC, Peehl, DM, Herr, AE: Microfluidic integration for automated targeted proteomic assays. proceedings of the national academy of sciences of the united states of america 109(16): 5972-5977, APR 17 2012.
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Selected Publications
b6 Gartner, Z, Hughes, A: MATERIALS SCIENCE Bioprinting in space and time. nature 572(7767): 38-39, AUG 1 2019.117 Hughes, AJ, Mornin, JD, Biswas, SK, Beck, LE, Bauer, DP, Raj, A, Bianco, S, Gartner, ZJ: Quanti.us: a tool for rapid, flexible, crowd-based annotation of images. nature methods 15(8): 587-+ AUG 2018.
13b Hughes, AJ, Miyazaki, H, Coyle, MC, Zhang, J, Laurie, MT, Chu, D, Vavrusova, Z, Schneider, RA, Klein, OD, Gartner, ZJ: Engineered Tissue Folding by Mechanical Compaction of the Mesenchyme. developmental cell 44(2): 165-+ JAN 22 2018.
f8 Hughes, AJ, Coyle, MC, Zhang, J, Gartner, ZJ: Tissue origami: Directed folding of tissues by programmed cell contractility networks. molecular biology of the cell 27, 2016.
126 Todhunter, ME, Jee, NY, Hughes, AJ, Coyle, MC, Cerchiari, A, Farlow, J, Garbe, JC, LaBarge, MA, Desai, TA, Gartner, ZJ: Programmed synthesis of three-dimensional tissues. nature methods 12(10): 975-981, OCT 2015.
d6 Hughes, AJ, Spelke, DP, Xu, ZC, Kang, CC, Schaffer, DV, Herr, AE: Single-cell western blotting. nature methods 11(7): 749-U94, JUL 2014.
100 Tentori, AM, Hughes, AJ, Herr, AE: Microchamber Integration Unifies Distinct Separation Modes for Two-Dimensional Electrophoresis. analytical chemistry 85(9): 4538-4545, MAY 7 2013.
f1 Hughes, AJ, Herr, AE: Microfluidic Western blotting. proceedings of the national academy of sciences of the united states of america 109(52): 21450-21455, DEC 26 2012.
138 Hughes, AJ, Tentori, AM, Herr, AE: Bistable Isoelectric Point Photoswitching in Green Fluorescent Proteins Observed by Dynamic Immunoprobed Isoelectric Focusing. journal of the american chemical society 134(42): 17582-17591, OCT 24 2012.
129 Hughes, AJ, Lin, RKC, Peehl, DM, Herr, AE: Microfluidic integration for automated targeted proteomic assays. proceedings of the national academy of sciences of the united states of america 109(16): 5972-5977, APR 17 2012.
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